Regulatory

Part:BBa_K1510100:Design

Designed by: Wei-Tai Chen   Group: iGEM14_NYMU-Taipei   (2014-10-06)


constitutive promoter (veg promoter) in S.mutans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)


Source

streptococcus mutans

References